Based on personal experience, provided you don't need single digit millisecond latency and the applications are not doing complicated things through DirectX for audio, you should have no issues using Wine. I've not done actual audio production with stuff in it, but I have gamed in it, and even a slight desync of the audio and video in a game can cause issues while playing, so I went to the trouble of actually measuring latency. In my tests, on my system, I got about 30ms latency relative to the same audio playback (same files, same software) running natively on Linux, though do note that that system was optimized to minimize latency, which is not the case for most distributions out there.
That said, even if it weren't for that, VirtualBox would almost certainly provide worse latency anyway.
With a native application, audio takes the following path (in most cases):
Application -> [Audio Library] -> [Sound Server] -> Sound Driver -> Hardware
With the audio library and sound server being functionally optional. So, natively, you have between 3 and 5 layers that your audio is going through before it gets to hardware.
With a Windows application running under Wine, you have the following:
Application -> [Audio Library -> [Audio Library]] -> Wine -> Sound Server -> Sound driver -> Hardware
With only the audio libraries between the app and Wine being optional. This means you have between 4 and 6 layers that your audio goes through before it gets to hardware.
With a Windows application running in Windows in Virtual Box (or another VM platform), you instead have the following monstrosity:
Application -> [Audio Library -> [Audio Library]] -> Windows Sound Driver -> VirtualBox Emulated Hardware -> Audio Library -> Sound Server -> Linux Sound Driver -> hardware
With only the audio libraries inside the virtual machine being functionally optional. So, for VirtualBox, you have between 6 and 8 layers your audio has to go through.
Note that this all assumes you primarily care about average latency, if you want to minimize variance in latency too, then things get way more complicated, but you still end up better off running natively (or failing that running under Wine) than with VirtualBox.