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I need to get this kind of information into numbers, how?

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  3. R's biOps here as a generic tool. R's PET has Hough transform here (but may not work in this domain but HT does not seem too complicated, here)

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There's the Haskell explore package meant to do that:

Very first prototype of a command line tool to extract pixel positions matching a given colour to help reconstruct plot data.

(Also see its homepage. I've never used this, though.)

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