From the manual:
-O ctl_cmd
Control an active connection multiplexing master process. When
the -O option is specified, the ctl_cmd argument is interpreted
and passed to the master process. Valid commands are: check
(check that the master process is running), forward (request
forwardings without command execution), cancel (cancel
forwardings), exit (request the master to exit), and stop
(request the master to stop accepting further multiplexing
requests).
Older versions only have check and exit, but that's enough for your purpose.
ssh -O check host.example.com
If you want to delete all connections (not just the connection to a particular host) in one fell swoop, then fuser /tmp/ssh_mux_* or lsof /tmp/ssh_mux_* will list the ssh clients that are controlling each socket. Use fuser -HUP -k tmp/ssh_mux_* to kill them all cleanly (using SIGHUP as the signal is best as it lets the clients properly remove their socket).
ssh -S none(maybe this helps you already). – sr_ Nov 5 '11 at 9:15